independent expression cassettes Search Results


mcherry expression cassette  (Promega)
90
Promega mcherry expression cassette
Effect of SLFN14 on viral replication. ( A ) Role of the RNA Polymerase III-RIG-I-IFN signaling pathway in the SLFN14 activity. HEK293T cells co-transfected with HIVluc expression plasmid and an empty plasmid (control cells) or a plasmid expressing human SLFN14 were treated or not with inhibitors indicated. Luciferase activity measured in these cells was expressed as % of control cells. Data correspond to a triplicate experiment and are representative of three independent experiments. Although not indicated, statistically significant differences ( p ≤ 0.0001) were found between the control and each of the other groups, as calculated with two-way ANOVA and Dunnett post-hoc tests. ( B ) Effect of SLFN14 on HIV-1 replication. ( I ) CD4 <t>and</t> <t>CXCR4</t> expression. HEK293T cells were co-transfected with plasmids expressing CD4 and a bicistronic plasmid encoding CXCR4 and <t>mCherry,</t> and either, an empty plasmid (control cells) or a human SLFN14 expression plasmid. CD4 was detected by immunostaining and MFI values of CD4 and mCherry (CXCR4) were quantified by flow cytometry. ( II ) These cells were infected with HIV-1 wild-type and viral replication was followed by measuring HIV-1 p24 in the cell supernatant. Data pertain to a triplicate experiment and they are representative of two independent experiments. Statistically significant differences were calculated with one-way ANOVA and Bonferroni post-hoc tests **** p ≤ 0.0001. Figure is created by Valenzuela et al.
Mcherry Expression Cassette, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/independent+expression+cassettes/pmc11054720-40-7-23?v=Promega
Average 90 stars, based on 1 article reviews
mcherry expression cassette - by Bioz Stars, 2026-08
90/100 stars
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i-scei restriction site-flanked expression cassettes  (Helmholtz Zentrum fur Infektionsforschung GmbH)
90
Helmholtz Zentrum fur Infektionsforschung GmbH i-scei restriction site-flanked expression cassettes
Effect of SLFN14 on viral replication. ( A ) Role of the RNA Polymerase III-RIG-I-IFN signaling pathway in the SLFN14 activity. HEK293T cells co-transfected with HIVluc expression plasmid and an empty plasmid (control cells) or a plasmid expressing human SLFN14 were treated or not with inhibitors indicated. Luciferase activity measured in these cells was expressed as % of control cells. Data correspond to a triplicate experiment and are representative of three independent experiments. Although not indicated, statistically significant differences ( p ≤ 0.0001) were found between the control and each of the other groups, as calculated with two-way ANOVA and Dunnett post-hoc tests. ( B ) Effect of SLFN14 on HIV-1 replication. ( I ) CD4 <t>and</t> <t>CXCR4</t> expression. HEK293T cells were co-transfected with plasmids expressing CD4 and a bicistronic plasmid encoding CXCR4 and <t>mCherry,</t> and either, an empty plasmid (control cells) or a human SLFN14 expression plasmid. CD4 was detected by immunostaining and MFI values of CD4 and mCherry (CXCR4) were quantified by flow cytometry. ( II ) These cells were infected with HIV-1 wild-type and viral replication was followed by measuring HIV-1 p24 in the cell supernatant. Data pertain to a triplicate experiment and they are representative of two independent experiments. Statistically significant differences were calculated with one-way ANOVA and Bonferroni post-hoc tests **** p ≤ 0.0001. Figure is created by Valenzuela et al.
I Scei Restriction Site Flanked Expression Cassettes, supplied by Helmholtz Zentrum fur Infektionsforschung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/independent+expression+cassettes/pm20735570-43-31-2?v=Helmholtz+Zentrum+fur+Infektionsforschung+GmbH
Average 90 stars, based on 1 article reviews
i-scei restriction site-flanked expression cassettes - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
gene exp mthfs hs00197574 m1  (Thermo Fisher)
85
Thermo Fisher gene exp mthfs hs00197574 m1
Effect of SLFN14 on viral replication. ( A ) Role of the RNA Polymerase III-RIG-I-IFN signaling pathway in the SLFN14 activity. HEK293T cells co-transfected with HIVluc expression plasmid and an empty plasmid (control cells) or a plasmid expressing human SLFN14 were treated or not with inhibitors indicated. Luciferase activity measured in these cells was expressed as % of control cells. Data correspond to a triplicate experiment and are representative of three independent experiments. Although not indicated, statistically significant differences ( p ≤ 0.0001) were found between the control and each of the other groups, as calculated with two-way ANOVA and Dunnett post-hoc tests. ( B ) Effect of SLFN14 on HIV-1 replication. ( I ) CD4 <t>and</t> <t>CXCR4</t> expression. HEK293T cells were co-transfected with plasmids expressing CD4 and a bicistronic plasmid encoding CXCR4 and <t>mCherry,</t> and either, an empty plasmid (control cells) or a human SLFN14 expression plasmid. CD4 was detected by immunostaining and MFI values of CD4 and mCherry (CXCR4) were quantified by flow cytometry. ( II ) These cells were infected with HIV-1 wild-type and viral replication was followed by measuring HIV-1 p24 in the cell supernatant. Data pertain to a triplicate experiment and they are representative of two independent experiments. Statistically significant differences were calculated with one-way ANOVA and Bonferroni post-hoc tests **** p ≤ 0.0001. Figure is created by Valenzuela et al.
Gene Exp Mthfs Hs00197574 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/independent+expression+cassettes/pm19636555-80-143--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp mthfs hs00197574 m1 - by Bioz Stars, 2026-08
85/100 stars
  Buy from Supplier

Image Search Results


Effect of SLFN14 on viral replication. ( A ) Role of the RNA Polymerase III-RIG-I-IFN signaling pathway in the SLFN14 activity. HEK293T cells co-transfected with HIVluc expression plasmid and an empty plasmid (control cells) or a plasmid expressing human SLFN14 were treated or not with inhibitors indicated. Luciferase activity measured in these cells was expressed as % of control cells. Data correspond to a triplicate experiment and are representative of three independent experiments. Although not indicated, statistically significant differences ( p ≤ 0.0001) were found between the control and each of the other groups, as calculated with two-way ANOVA and Dunnett post-hoc tests. ( B ) Effect of SLFN14 on HIV-1 replication. ( I ) CD4 and CXCR4 expression. HEK293T cells were co-transfected with plasmids expressing CD4 and a bicistronic plasmid encoding CXCR4 and mCherry, and either, an empty plasmid (control cells) or a human SLFN14 expression plasmid. CD4 was detected by immunostaining and MFI values of CD4 and mCherry (CXCR4) were quantified by flow cytometry. ( II ) These cells were infected with HIV-1 wild-type and viral replication was followed by measuring HIV-1 p24 in the cell supernatant. Data pertain to a triplicate experiment and they are representative of two independent experiments. Statistically significant differences were calculated with one-way ANOVA and Bonferroni post-hoc tests **** p ≤ 0.0001. Figure is created by Valenzuela et al.

Journal: Viruses

Article Title: Schlafen14 Impairs HIV-1 Expression in a Codon Usage-Dependent Manner

doi: 10.3390/v16040502

Figure Lengend Snippet: Effect of SLFN14 on viral replication. ( A ) Role of the RNA Polymerase III-RIG-I-IFN signaling pathway in the SLFN14 activity. HEK293T cells co-transfected with HIVluc expression plasmid and an empty plasmid (control cells) or a plasmid expressing human SLFN14 were treated or not with inhibitors indicated. Luciferase activity measured in these cells was expressed as % of control cells. Data correspond to a triplicate experiment and are representative of three independent experiments. Although not indicated, statistically significant differences ( p ≤ 0.0001) were found between the control and each of the other groups, as calculated with two-way ANOVA and Dunnett post-hoc tests. ( B ) Effect of SLFN14 on HIV-1 replication. ( I ) CD4 and CXCR4 expression. HEK293T cells were co-transfected with plasmids expressing CD4 and a bicistronic plasmid encoding CXCR4 and mCherry, and either, an empty plasmid (control cells) or a human SLFN14 expression plasmid. CD4 was detected by immunostaining and MFI values of CD4 and mCherry (CXCR4) were quantified by flow cytometry. ( II ) These cells were infected with HIV-1 wild-type and viral replication was followed by measuring HIV-1 p24 in the cell supernatant. Data pertain to a triplicate experiment and they are representative of two independent experiments. Statistically significant differences were calculated with one-way ANOVA and Bonferroni post-hoc tests **** p ≤ 0.0001. Figure is created by Valenzuela et al.

Article Snippet: The CXCR4 expression plasmid contains an independent mCherry expression cassette (bicistronic plasmid). pCI Luc contains firefly luciferase cDNA cloned MluI/Xba I in pCI (Promega, Madison, WI, USA). pNLENG1-ES-IRES (a gift of D.N.

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Control, Luciferase, Immunostaining, Flow Cytometry, Infection